Last One Laughing UK's Season 2 line-up finally unveiled by Prime Video

*Warning: Contains spoilers for the first season of Last One Laughing UK*

Prime Video has just unveiled the 10 comedians taking part in the second series of Last One Laughing UK - and it could be bad news for the returning champion.

The series places 10 comics from all disciplines in a room for a day with one simple instruction: do not laugh. The simple but effective show has proven to be a huge hit for Prime Video, with a second season given the green light shortly after the first debuted in March this year.

Bob Mortimer sailed through to the final of the first season, finding himself going head-to-head with Richard Ayoade in the battle to keep a straight face. Ultimately, Bob managed to elicit a chuckle from the IT Crowd star and was crowned the first ever winner.

Now the streaming giant has announced which fresh faces will be taking part in Season 2, and Bob faces some stiff competition.

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Joining the reigning champion is David Mitchell; Diane Morgan; Romesh Ranganathan; Amy Gledhill; Maisie Adam; Mel Giedroyc; Alan Carr; Gbemisola Ikumelo and Sam Campbell. Meanwhile Jimmy Carr will return as host of the programme alongside his faithful deputy Roisin Conaty.

Prime Video's synopsis for the upcoming season teases: "In this new series, Jimmy Carr will once again challenge 10 of Britain’s funniest comics to spend the day together without so much as cracking a smile. Over the course of the series, they will use every ounce of their comedic talents to try and break their opponents without cracking up.

"And it’s not just their rivals they need to watch out for. The show is packed with comedy cameos, format twists and surprises designed to elicit laughs from both players and viewers alike. The rules are simple: laugh and you’re out."

The series is an adaptation of an Amazon Original from Japan called Documental in which 10 comedians bet their own money in a "battle of laughter behind closed doors." It spawned the Last One Laughing format, which has now been replicated in over 20 territories including an Irish version hosted by Graham Norton and a South African one with Trevor Noah at the helm.

The first UK series saw Bob Mortimer compete alongside Daisy May Cooper; Joe Lycett; Judi Love; Rob Beckett; Sara Pascoe; Lou Sanders; Harriet Kemsley; Richard Ayoade and Joe Wilkinson.

It resulted in some hilarious moments, including Bob's 'Slender Ladies' song and Joe's RNLI speech that has since amassed hundreds of thousands of views on YouTube.

"It doesn't matter how many times I watch this, I will never get through it without laughing. I don't know how he didn't take them all out. PERFECTLY pitched for the occasion. The delivery is magnificent," said one fan. "I’ve seen this about 6 times and I still can’t help but laugh," said another of the clip.

Last One Laughing UK Season 2 comes to Prime Video in 2026